| 质粒名称: | pHR_5x Gal4 UAS |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 79119 |
| 存储实验室: | Wendell Lim |
|
载体骨架基本信息 |
|
| 载体名称: | pHR_5x Gal4 UAS |
| 空载体大小: | 4038 bp |
| 完整质粒大 | 8689bp |
| 载体修饰: | pHR |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄青霉素, 100 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
文献引用方法 |
|
| 材料和方法部分: | pHR_5x Gal4 UAS was a gift from Wendell Lim (Addgene plasmid # 79119 ; http://n2t.net/addgene:79119 ; RRID:Addgene_79119) |
| 参考文献部分: | Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Morsut L, Roybal KT, Xiong X, Gordley RM, Coyle SM, Thomson M, Lim WA. Cell. 2016 Feb 11;164(4):780-91. doi: 10.1016/j.cell.2016.01.012. Epub 2016 Jan 28. 10.1016/j.cell.2016.01.012 PubMed 26830878 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pHR_5x Gal4 UAS 8689 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Base vector for cloning Gal4DBDVP64 inducible genes.
ACCESSION .
VERSION .
KEYWORDS pHR_5x Gal4 UAS
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8689)
AUTHORS Morsut L, Roybal KT, Xiong X, Gordley RM, Coyle SM, Thomson M, Lim
WA
TITLE Engineering Customized Cell Sensing and Response Behaviors Using
Synthetic Notch Receptors.
JOURNAL Cell. 2016 Feb 11;164(4):780-91. doi: 10.1016/j.cell.2016.01.012.
Epub 2016 Jan 28.
PUBMED 26830878
REFERENCE 2 (bases 1 to 8689)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8689
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 61..294
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
promoter complement(319..337)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(320..337)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(627..646)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind 980..1002
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(1040..1058)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 1126..1230
/gene="bla"
/label=AmpR promoter
CDS 1231..2091
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(1449..1468)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 2262..2850
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2751..2770
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 3004..3021
/label=L4440
/note="L4440 vector, forward primer"
primer_bind complement(3091..3111)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 3096..3425
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3276..3411
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3338..3357
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
intron 4559..4624
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 4754..4774
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 5199..5333
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5236..5255)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5290..5309
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
LTR 5502..6135
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 6182..6307
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 6799..7032
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 7217..7261
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 7546..7663
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
primer_bind 7889..7913
/label=LNCX
/note="Human CMV promoter, forward primer"
misc_feature 8099..8687
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(8152..8172)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(8570..8581)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
ORIGIN
1 cgataccgtc gaccaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
61 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
121 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
181 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
241 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatct
301 agaattaatt ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga tacataaggt
361 tatgtattaa ttgtagccgc gttctaacga caatatgtac aagcctaatt gtgtagcatc
421 tggcttactg aagcagaccc tatcatctct ctcgtaaact gccgtcagag tcggtttggt
481 tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc
541 aatcagcagg gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg ccggcatcac
601 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg
661 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt
721 ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct
781 caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg
841 tcgaatggtg cactctcagt acaatctagc tctgatgccg catagttaag ccagccccga
901 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
961 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
1021 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
1081 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
1141 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
1201 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
1261 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
1321 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
1381 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
1441 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
1501 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
1561 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
1621 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
1681 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
1741 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
1801 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
1861 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
1921 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
1981 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
2041 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
2101 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
2161 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
2221 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
2281 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
2341 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
2401 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
2461 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
2521 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
2581 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
2641 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
2701 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
2761 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
2821 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
2881 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
2941 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
3001 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
3061 gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt
3121 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
3181 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
3241 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
3301 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
3361 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
3421 gcaaaaagct tggacacaag acaggcttgc gagatatgtt tgagaatacc actttatccc
3481 gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt
3541 gcgccagatc tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta
3601 gataaacagg ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg
3661 cagatccatg cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt
3721 attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc
3781 gtcatgtcga taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag
3841 tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata
3901 ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct
3961 tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata
4021 cctggattga acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct
4081 aatcttttca acgcctggca ctgccgggcg ttgttctttt taacttcagg cgggttacaa
4141 tagtttccag taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc
4201 ctgttcaaac cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac
4261 ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa ccatccctca ctggtatcgc
4321 atgattaacc gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc
4381 caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg atgatttata cgatacggtg
4441 attggctacc gtggcggcaa ctggatttat gagtgggccc cggatctttg tgaaggaacc
4501 ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt
4561 aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt
4621 ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga
4681 aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca
4741 acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga
4801 attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat
4861 ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt
4921 aacctttata agtaggcata acagttataa tcataacata ctgttttttc ttactccaca
4981 caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt
5041 aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa
5101 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
5161 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
5221 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
5281 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga
5341 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca
5401 attacctagt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa
5461 agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc
5521 ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
5581 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta
5641 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag
5701 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg
5761 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt
5821 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag
5881 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc
5941 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
6001 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
6061 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
6121 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
6181 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
6241 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
6301 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
6361 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
6421 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
6481 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
6541 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
6601 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg gtgatcttca
6661 gacctggacg atatatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
6721 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
6781 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
6841 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
6901 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
6961 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
7021 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
7081 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
7141 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
7201 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
7261 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
7321 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
7381 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
7441 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
7501 gaacggatct cgacggtatc gccaaatggc agtattcatc cacaatttta aaagaaaagg
7561 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
7621 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
7681 cagcagagat ccagtttgga tcgataagct tgatatcgaa ttcggagcac tgtcctccga
7741 acgtcggagc actgtcctcc gaacgtcgga gcactgtcct ccgaacgtcg gagcactgtc
7801 ctccgaacgg agcatgtcct ccgaacgtcg gagcactgtc ctccgaacga ctagttaggc
7861 gtgtacggtg ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga
7921 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctctcga
7981 cattcgttgg atccttgact tgcggccgca actcccacct gcaacatgcg tgactgactg
8041 aggccgcgac tctagagtcg acctgcaggc atgcaagctt gatatcaagc ttatcgataa
8101 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
8161 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
8221 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
8281 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
8341 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
8401 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
8461 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
8521 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
8581 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
8641 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcat
//
